Interaction of eosinophils and dermal fibroblasts in fungal-infected atopic dermatitis
Refereed conference paper presented and published in conference proceedings


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摘要Atopic dermatitis (AD) is an allergic inflammatory skin disease, histologically characterized by
eosinophils infiltration in the dermal layer. This chronic and pruritus disease is often aggravated by
microbial infection in the dried and impaired skin area. Colonization of cutaneous fungal communities
such as Malassezia yeast and Candida yeast has been reported in AD patients. Nevertheless, the
interaction between the infiltrating effector cells eosinophils, human dermal fibroblasts (HDF) and the
yeast is not well-understood. Therefore, we aim at investigating the immunological mechanism
underlying the fungal interaction with eosinophils in the skin of AD.

Dectin-1 agonists curdlan and heat-killed Candida albicans (HKCA), as well as the dectin-2 agonist
furfurman, were co-cultured with the human eosinophils and HDF. Inflammatory cytokines and
adhesion molecules were measured by cytometric bead-based assay and flow cytometry, respectively.
Upon curdlan stimulation, the release of AD-related IL-6, CXCL8, TNF-α and CCL2 were significantly
upregulated in eosinophils/HDF co-culture but not in eosinophils single culture. Adhesion molecule
ICAM-1 and VCAM-1 were also expressed on both cells. IL-6 and CXCL8 were exclusively increased
in the co-culture system upon HKCA stimulation. Dectin-2 was expressed on eosinophils but not on
HDF. In eosinophils/HDF co-culture, the release of IL-6, CXCL8 and CCL5 were significantly
increased.

The increase in pro-inflammatory cytokines and adhesion molecules expression highlight the
pathological immune response in the dermal layer in AD. The results demonstrated the interaction
between the skin and eosinophils upon fungal colonization. Further experiments are ongoing to
evaluate the underlying mechanism of fungal infection in AD.
出版社接受日期19.07.2019
著者Miranda Sin-Man TSANG, Chun-Kwok WONG
會議名稱17th International Congress of Immunology of the International-Union-of-Immunological-Societies (IUIS)
會議開始日19.10.2019
會議完結日23.10.2019
會議地點Beijing
會議國家/地區中國
會議論文集題名European Journal of Immunology
出版年份2019
月份10
卷號49
期次Suppl 3
頁次737 - 738
國際標準期刊號0014-2980
語言英式英語

上次更新時間 2020-15-11 於 00:02