Interaction of eosinophils and dermal fibroblasts in fungal-infected atopic dermatitis
Refereed conference paper presented and published in conference proceedings


Full Text

Times Cited
Web of Science0WOS source URL (as at 08/07/2020) Click here for the latest count

Other information
AbstractAtopic dermatitis (AD) is an allergic inflammatory skin disease, histologically characterized by
eosinophils infiltration in the dermal layer. This chronic and pruritus disease is often aggravated by
microbial infection in the dried and impaired skin area. Colonization of cutaneous fungal communities
such as Malassezia yeast and Candida yeast has been reported in AD patients. Nevertheless, the
interaction between the infiltrating effector cells eosinophils, human dermal fibroblasts (HDF) and the
yeast is not well-understood. Therefore, we aim at investigating the immunological mechanism
underlying the fungal interaction with eosinophils in the skin of AD.

Dectin-1 agonists curdlan and heat-killed Candida albicans (HKCA), as well as the dectin-2 agonist
furfurman, were co-cultured with the human eosinophils and HDF. Inflammatory cytokines and
adhesion molecules were measured by cytometric bead-based assay and flow cytometry, respectively.
Upon curdlan stimulation, the release of AD-related IL-6, CXCL8, TNF-α and CCL2 were significantly
upregulated in eosinophils/HDF co-culture but not in eosinophils single culture. Adhesion molecule
ICAM-1 and VCAM-1 were also expressed on both cells. IL-6 and CXCL8 were exclusively increased
in the co-culture system upon HKCA stimulation. Dectin-2 was expressed on eosinophils but not on
HDF. In eosinophils/HDF co-culture, the release of IL-6, CXCL8 and CCL5 were significantly
increased.

The increase in pro-inflammatory cytokines and adhesion molecules expression highlight the
pathological immune response in the dermal layer in AD. The results demonstrated the interaction
between the skin and eosinophils upon fungal colonization. Further experiments are ongoing to
evaluate the underlying mechanism of fungal infection in AD.
Acceptance Date19/07/2019
All Author(s) ListMiranda Sin-Man TSANG, Chun-Kwok WONG
Name of Conference17th International Congress of Immunology of the International-Union-of-Immunological-Societies (IUIS)
Start Date of Conference19/10/2019
End Date of Conference23/10/2019
Place of ConferenceBeijing
Country/Region of ConferenceChina
Proceedings TitleEuropean Journal of Immunology
Year2019
Month10
Volume Number49
Issue NumberSuppl 3
Pages737 - 738
ISSN0014-2980
LanguagesEnglish-United Kingdom

Last updated on 2020-09-07 at 00:58