High-throughput immunological marker analysis of Dictamni Cortex: Application in the quality control of herbal medicine for inflammatory diseases
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AbstractBackground: Quality inconsistency of herbal medicine is an obstacle that limits the extensive use and
study of traditional Chinese medicine (TCM). Differences in environmental conditions and processing
methods of herbal medicine often result in varying clinical outcomes in patients. Standard chemical
markers evaluated for the quality control (QC) of herbal medicines may not be therapeutically relevant;
therefore, they cannot comprehensively reflect the biological quality of the herbs. ln view of this, a novel
QC method for a better assessment of herbal medicine has been developed using immunological markers.

Methods: Immunological activities of Dictamni Cortex (DC), a typical herbal medicine for treating
various inflammatory diseases. produced from two regions of Inner Mongolia" Hebei as well as Anhui,
were evaluated. Upon in vitro treatment with their water and ethanol extracts, differential patterns of
inflammatory cytokine TNF-a, IL-10. IL-6 and IL-lb and chemokine CXCL5 released from the
lipopolysaccharides (LPS)- and/or phytohaemagglutinin (PHA)-stimulated human peripheral blood
mononuclear cells (PBMC) were measured by multiplex bead-based array assays.

Results: Based on the macroscopic features and the thin-layer chromatography results, the four tested
DC crude drug samples fulfilled the authentication requirements as specified in current The Chinese
Pharmacopoeia (2015 Edition). Ultra-performance liquid chromatography and liquid chromatography mass spectrometry also revealed that all four DC samples contained the required chemical standard
obacunone and fraxinellone. However. distinct cytokine and chemokine patterns were observed among
the four DC ethanol extracts upon PHA-only, as well as upon LPS and PHA co-stimulation. Nevertheless,
the patterns were largely similar among the water / ethanol extracts upon LPS-only stimulation The
endotoxin level, heavy metals, toxic element and pesticides residues content in the four DC crude
samples were minimal.

Conclusion & Implications: Dictamni Cortex that originated from different geographical locations of
China shows distinct anti-inflammatory activities in vitro. In addition to the commonly used
morphological, chemical or DNA markers, the novel high-throughput inflammatory cytokine and
chemokine profiles released by human PBMC upon treatment with herbal extracts could be an important
reference to help for the quality control of anti-inflammatory TCM in the future.
All Author(s) ListMiranda Sin-Man TSANG, Pang-Chui SHAW, Ida Miu-Ting CHU, Ling CHENG, Eric Chun-Wai WONG, David Tai-Wai LAU, Christopher Wai-Kei LAM, Chun-Kwok WONG
Name of ConferenceHong Kong Society for Immunology 2019 Annual General Meeting and Scientific Meeting
Start Date of Conference12/10/2019
End Date of Conference12/10/2019
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Year2019
Month10
LanguagesEnglish-United Kingdom

Last updated on 2020-22-04 at 10:32