Flavonoids of Sophora flavescens promote autophagy and anti-inflammatory activities in Bacillus Calmette-Guérin-stimulated murine macrophages
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摘要Tuberculosis (TB), the most infectious air-borne diseases, threatens global health. Conventional antibiotic treatment and Mycobacterium bovis-attenuated Bacilli Calmette-Guerin (BCG) vaccines may cause side effects, in which adjunct therapy may be necessary. Flavonoids in medicinal herb Sophora Flavescens (FSF) have been shown to suppress inflammation and bacterial proliferation in TB infection. Autophagy and anti-inflammatory mechanisms of FSF in TB infection, and molecular and cellular characterizations of FSF in BCG-infected macrophages are examined.

Murine alveolar macrophage cell line MH-S cells and primary murine peritoneal cells were stimulated in vitro with heat-inactivated BCG and treated with Flavonoids of Sophora Flavescens (FSF), with or without autophagy inhibitor Bafilomycin A1. Autophagy-related gene expression and cytokine release was measured with quantitative PCR and Milliplex assay, respectively, to assess the extent of autophagy activation in BCG-simulated macrophages. Autophagy-related proteins were measured using western blot, flow cytometry and fluorescent microscopy.

Results showed that autophagy-related protein LC3 and P62, and gene AMBRA1, FBXL20 and RAB24, were upregulated significantly following FSF treatment in both BCG-stimulated MH-S cells and primary murine macrophages. BCG-induced pro-inflammatory cytokines such as CCL2 and IL-6 were suppressed upon FSF treatment, which could be reversed by Bafilomycin A1. Therefore, FSF can suppress the inflammatory response in BCG-stimulated MH-S cells via the upregulation of autophagy process, however the interplay between anti-inflammation and autophagy needs further evaluation.
出版社接受日期13.09.2019
著者Kan Ling Yu Lea, Liu Dehua, Chan Chung Lap, Leung Ping Chung, Wong Chun Kwok
會議名稱Japanese Society for Immunology 48th Annual Meeting 2019 (JSI 2019)
會議開始日11.12.2019
會議完結日13.12.2019
會議地點Hamamatsu
會議國家/地區日本
出版年份2019
語言英式英語

上次更新時間 2020-26-05 於 12:21