Synthetic Multienzyme Complexes, Catalytic Nanomachineries for Cascade Biosynthesis In Vivo
Publication in refereed journal

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其它資訊
摘要Multienzyme complexes, or metabolons, are assemblies or clusters of sequential enzymes that naturally exist in metabolic pathways. These nanomachineries catalyze the conversion of metabolites more effectively than the freely floating enzymes by minimizing the diffusion of intermediates in vivo. Bioengineers have devised synthetic versions of multienzyme complexes in cells to synergize heterologous biosynthesis, to improve intracellular metabolic flux, and to achieve higher titer of valuable chemical products. Here, we utilized orthogonal protein reactions (SpyCatcher/SpyTag and SnoopCatcher/SnoopTag pairs) to covalently assemble three key enzymes in the mevalonate biosynthesis pathway and showed 5-fold increase of lycopene and 2-fold increase of astaxanthin production in Escherichia coli. The multienzyme complexes are ellipsoidal nanostructures with hollow interior space and uniform thickness and shapes. Intracellular covalent enzyme assembly has yielded catalytic nanomachineries that drastically enlarged the flux of carotenoid biosynthesis in vivo. These studies also deepened our understanding on the complexity of hierarchical enzyme assembly in vivo.
出版社接受日期29.07.2019
著者Jiale Qu, Sheng Cao, Qixin Wei, Huawei Zhang, Rui Wang, Wei Kang, Tian Ma, Liang Zhang, Tiangang Liu, Shannon Wing-Ngor Au, Fei Sun, Jiang Xia
期刊名稱ACS Nano
出版年份2019
月份9
日期24
卷號13
期次9
出版社ACS
頁次9895 - 9906
國際標準期刊號1936-0851
語言美式英語

上次更新時間 2020-27-10 於 23:34