The PinX1/NPM interaction associates with hTERT in early-S phase and facilitates telomerase activation
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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摘要Background: Telomere maintenance is an important factor in tumorigenesis. PinX1 is a potent telomerase regulator which also involves in telomerase loading to telomeres. Nucleophosmin (NPM) can partially attenuate PinX1 inhibition of telomerase activity and NPM loading to hTERT requires PinX1. However, the role of the PinX1/NPM interaction in telomerase activity is not fully understood. Method: The long-term effects of PinX1 and NPM down-regulation on telomere length were investigated by TRF assay. The localization of the PinX1/NPM association and the NPM/PinX1/hTERT complex formation were examined by immunofluorescence studies. Results: Concurrent long-term down-regulation of PinX1 and NPM led to a substantial decrease in telomere length. The interaction with PinX1 was crucial in NPM localization in the nucleolus during the S phase. PinX1 and NPM associated throughout S phase and the NPM/PinX1/hTERT complex formation peaked during the early-S phase. The PinX1/NPM interaction was shown to localize away from Cajal Bodies at the start of S phase. Conclusion: PinX1/NPM interaction is important in telomerase regulation during catalysis. NPM is recruited to hTERT by PinX1 and is required in the proposed telomerase modulating unit to activate telomerase when telomere extension occurs during S phase.
出版社接受日期13.06.2019
著者Sai-Tim Ho, Rui Jin, Derek Hang-Cheong Cheung, Jun-Jian Huang, Pang-Chui Shaw
期刊名稱Cell and Bioscience
出版年份2019
月份6
日期13
卷號9
出版社BMC
文章號碼47
國際標準期刊號2045-3701
語言英式英語

上次更新時間 2020-25-09 於 12:22