Mitochondrial dynamics and biogenesis in the maturation of embryonic stem cell-derived cardiomyocytes
Refereed conference paper presented and published in conference proceedings



摘要Embryonic stem cells (ESCs) can differentiate into cardiomyocytes. This could provide a promising source of cells for drug development, regenerative therapy and developmental biology study. However, these cardiomyocytes are immature; they do not fully possess morphological and functional characteristics of adult cardiomyocytes. In order to get functional cardiomyocytes which could be applied for further studies, the mechanism underlying the maturation of mouse ESC (mESC)-derived cardiomyocytes (mESC-CMs) has to be better understood. Mitochondria occupy higher volume of adult cardiomyocytes compared with immature cardiomyocytes and become longer and bigger through the maturation process of the cardiomyocytes. What is more, mitochondria distribution is getting more regular in “crystal-like” pattern along the sarcomere in adult cardiomyocytes while it is in a rectangular network in immature cardiomyocytes. There are evidence showing that mitochondrial biogenesis and dynamics together regulate the structure and content of mitochondria. Since it has been reported that intervention on mitochondrial biogenesis and dynamics could influence mESC differentiation, we speculate that changing mitochondrial biogenesis and/or dynamics can affect the maturation of mESC-derived cardiomyocytes. In this project, we found that there was a higher cytosolic occupancy of mitochondria in late-stage mESC-CMs compared with early-stage mESC-CMs. We will investigate whether we could promote the maturation of mESC-CMs through manipulating mitochondrial biogenesis and/or dynamics. The maturation assessment system will be established in terms of morphology, marker genes expression, calcium handling ability and electrophysiology.
著者Yanxiang Qi, Suk Ying Tsang
會議名稱The Hong Kong Inter-University Postgraduate Symposium in Biochemical Sciences 2019
會議地點Hong Kong
會議論文集題名The Hong Kong Inter-University Postgraduate Symposium in Biochemical Sciences 2019

上次更新時間 2020-20-05 於 10:50