Potential role of transient receptor potential ankyrin 1 channels in mouse embryonic stem cell-derived cardiomyocytes
Refereed conference paper presented and published in conference proceedings



摘要Transient receptor potential (TRP) channels are broadly expressed in a variety of tissues and cell types. They are able to respond to a wide range of stimuli in the cellular environment, which makes them act as cellular vanguard sensors involved in nociception, taste perception, temperature and osmolarity sensation. Among TRP channels, TRP ankyrin 1 (TPRA1) channel was initially described as a cold-sensitive non-selective cation channel expressed in neurons. Recently, emerging evidence indicates that TRPA1 is expressed in various cell types including cardiomyocytes (CMs) and plays an important role in CM contractile function. Mouse embryonic stem cells (mESCs) are able to self-renew and maintain pluripotency to differentiate into all cell lineages including CMs. In CMs, mitochondria can not only supply energy to cells but also has a key role in the regulation of calcium homeostasis and cell contraction. The function of mitochondrion is tightly related to its morphology, which is determined by continuous fission and fusion, called mitochondrial dynamics. Up till now, there is limited knowledge on how TRPA1 regulates intracellular calcium ([Ca2+]i) and action potential in CMs and whether TRPA1 exerts an effect on mitochondria function. Our preliminary results indicated that mESC-derived CMs (mESC-CMs) expressed TRPA1. In addition, we found that the activities of TRPA1 are positively associated with the Ca2+ transients (CaTs) in mESC-CMs. Moreover, TRPA1 activator increased mitochondrial fusion while TRPA1 blocker increased mitochondrial fission. However, the exact mechanism is still under investigation. In the future, our research will focus on elucidating how the TRPA1 activity influences mitochondrial dynamics and their effects on the function of mESC-CMs.
著者Qianqian Ding, Suk Ying Tsang
會議名稱International Society for Stem Cell Research Annual Meeting 2019
會議地點Los Angeles
會議論文集題名ISSCR 2019 Annual Meeting

上次更新時間 2020-20-05 於 10:39