Acute exposure to Triphenyl Phosphate inhibits the proliferation and cardiac differentiation of mouse embryonic stem cells and zebrafish
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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其它資訊
摘要Attention has recently paid to the interaction of triphenyl phosphate (TPHP) and body tissues, particularly within the reproductive and development systems, due to its endocrine-disrupting properties. However, the acute effects of TPHP on early embryonic development remain unclear. Here, we used mouse embryonic stem cells (mESC) and zebrafish embryos to investigate whether TPHP is an embryo toxicant. First, we found that continuous exposure of TPHP decreased the proliferation and increased the apoptotic populations of mESCs in a concentration-dependent manner. Results of mass spectrometry showed that the intracellular concentration of TPHP reached 39.45 ± 7.72 µg/g w/w after 3 hr of acute exposure with TPHP (38.35 μM) but gradually decreased from 3 hr to 48 hr. Additionally, DNA damage was detected in mESCs after a short-term treatment with TPHP, which in turn, activated DNA damage responses, leading to cell cycle arrest by changing the expression levels of p53, proliferating cell nuclear antigen, and Y15-phosphorylated Cdk I. Furthermore, our results revealed that short-term treatment with TPHP disturbed cardiac differentiation by decreasing the expression levels of Oct4, Sox2, and Nanog and transiently reduced the glycolysis capacity in mESCs. In zebrafish embryos, exposure to TPHP resulted in broad, concentration-dependent developmental defects and coupled with heart malformation and reduced heart rate. In conclusion, the two models demonstrate that acute exposure to TPHP affects early embryonic development and disturbs the cardiomyogenic differentiation.
著者Zenghua Qi, Min Chen, Yuanyuan Song, Xiya Wang, Bingkun Li, Zhi‐Feng Chen, Suk Ying Tsang, Zongwei Cai
期刊名稱Journal of Cellular Physiology
出版年份2019
月份11
卷號234
期次11
出版社Wiley
出版地USA
頁次21235 - 21248
國際標準期刊號0021-9541
電子國際標準期刊號1097-4652
語言美式英語

上次更新時間 2020-22-09 於 01:08