Chemokine (C-X-C motif) ligand 14 (CXCL14) impairs pancreatic beta cell function
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AbstractBackground and aims: Pancreatic beta cell dysfunction and insulin resistance are important factors in the pathophysiology of type 2 diabetes (T2D). Chemokines are chemo-attractants which guide the migration of inflammatory cells, especially macrophages, and have been shown to induce beta cell dysfunction. People with T2D or type 1 diabetes have higher serum levels of chemokines than normal individuals. The chemokine, CXCL12 (also known as SDF-1), and its receptor CXCR4, are crucial for beta cell differentiation, survival, and pancreatic islet genesis. However, these biological effects can be antagonized by CXCL14 which shares similar structure as CXCL12. Although CXCL14 knockout mice are protected from obesity-induced insulin resistance, whether CXCL14 may cause pancreatic beta cell dysfunction in the context of T2D has not been explored. Here, we aimed to examine the effects of CXCL14 on beta cell function.

Materials and methods: MIN6 cells, a pancreatic beta cell line derived from mouse insulinoma cells, and primary islets from C57/BL6 mice were treated with 100 ng/ml CXCL14 for different time points Expression of marker genes and total insulin production and glucose-stimulated insulin secretion (GSIS) were measured at different timepoints.

Results: In primary islets, CXCL14 impaired GSIS and total insulin production. CXCL14 treatment downregulated expression of genes related to insulin synthesis, including Pdx1, Isl1, Pax-6, MafA, Ins2, and genes involved in insulin secretion, including ZnT8, in both MIN6 cells and primary islets. In MIN6 cells, CXCL14 also reduced the expression of CXCL12 and CXCR4 and increased the phosphorylation of MAPK, ERK, and JNK in a time-dependent manner. CXCL14 activated MAPK which upregulated phosphorylation of ERK and JNK followed by activation of their downstream targets. CXCL14 also induced caspase 3 cleavage, suggesting a possible pro-apoptotic effect on beta cells.

Conclusion: CXCL14 impairs insulin synthesis and secretion and may be a potential target for treatment of T2D.
Acceptance Date19/08/2019
All Author(s) ListCAO Huanyi, TIAN Xiaoyu, CHUNG Chi Kong Arthur, MING Xing, LEE Heung Man, CHAN Chung Ngor Juliana, KONG Pik Shan
Name of ConferenceEast meets west symposium 2019
Start Date of Conference28/09/2019
End Date of Conference29/09/2019
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Year2019
Month9
LanguagesEnglish-United States

Last updated on 2020-14-05 at 17:01