High-throughput immunological marker analysis of Dictamni Cortex: Application in the quality control of herbal medicine for inflammatory diseases
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AbstractQuality inconsistency of herbal medicine is an obstacle that limits the extensive use and study
of traditional Chinese medicine (TCM). Differences in environmental conditions and
processing methods of herbal medicine often result in varying clinical outcomes in patients.
The standard chemical markers evaluated for the quality control (QC) of herbal medicines
may not be therapeutically relevant; therefore, they cannot comprehensively reflect the
biological quality of the herbs. In view of this, a novel QC method for better assessment of
herbal medicine has been developed using immunological markers. Immunological activities
of Dictamni Cortex, a typical herbal medicine for the treatment of various inflammatory
diseases, from different places of origin in China were evaluated using multiplex bead-based
array assays. Upon in vitro treatment of their water and ethanol extracts, differential patterns
of inflammatory cytokine TNF-��, IL-10, IL-6, and IL-1�� and chemokine CXCL8 were
released from the lipopolysaccharides- and/or phytohaemagglutinin-stimulated human
peripheral blood mononuclear cells (PBMC). Distinct patterns of TNF-�� and CXCL8 levels
were observed upon treatment of PBMC with Dictamni Cortex samples from different
geographical locations in China. Thus, in addition to the commonly used morphological,
chemical or DNA markers, the novel high-throughput inflammatory cytokine and chemokine
profiles released by PBMC upon treatment with herbal extracts could be an important
reference to help for the quality control of herbal medicine in the future.
All Author(s) ListMiranda Sin-Man TSANG, Pang-Chui SHAW, Ida Miu-Ting CHU, Ling CHENG, Eric Chun-Wai WONG, David Tai-Wai LAU, Christopher Wai-Kei LAM, Chun-Kwok WONG
Name of Conference24th Annual Scientific Meeting, Hong Kong Society of Flow Cytometry
Start Date of Conference09/03/2019
End Date of Conference09/03/2019
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Year2019
Month3
LanguagesEnglish-United Kingdom

Last updated on 2020-22-04 at 10:27