Analysis of spatiotemporal specificity of small RNAs regulating hPSC differentiation and beyond
Publication in refereed journal

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其它資訊
摘要We present a quantitative analysis of small RNA dynamics during the transition from hPSCs to the three germ layer lineages to identify spatiotemporal-specific small RNAs that may be involved in hPSC differentiation. To determine the degree of spatiotemporal specificity, we utilized two algorithms, namely normalized maximum timepoint specificity index (NMTSI) and across-tissue specificity index (ASI). NMTSI could identify spatiotemporal-specific small RNAs that go up or down at just one timepoint in a specific lineage. ASI could identify spatiotemporal-specific small RNAs that maintain high expression from intermediate timepoints to the terminal timepoint in a specific lineage. Beyond analyzing single small RNAs, we also quantified the spatiotemporal-specificity of microRNA families and observed their differential expression patterns in certain lineages. To clarify the regulatory effects of group miRNAs on cellular events during lineage differentiation, we performed a gene ontology (GO) analysis on the downstream targets of synergistically up-and downregulated microRNAs. To provide an integrated interface for researchers to access and browse our analysis results, we designed a web-based tool at https://keyminer.pythonanywhere.com/km/.
著者Lu LI, Jin Feng LI, Dan Dan CAO, Vassilios PAPADOPOULOS, Wai Yee CHAN
期刊名稱bioRxiv
出版年份2019
月份9
日期27
出版社Cold Spring Harbor Laboratory
電子國際標準期刊號2379-3708
語言美式英語

上次更新時間 2020-21-09 於 00:19