Reference-Based Identification of Long Noncoding RNAs in Plants with Strand-Specific RNA-Sequencing Data
Publication in refereed journal


摘要Long noncoding RNAs (lncRNAs) have been shown to play important roles in various organisms, including plant species. Several tools and pipelines have emerged for lncRNA identification, including reference-based transcriptome assembly pipelines and various coding potential calculating tools. In this protocol, we have integrated some of the most updated computational tools and described the procedures step-by-step for identifying lncRNAs from plant strand-specific RNA-sequencing datasets. We will start from clean RNA-sequencing reads, followed by reference-based transcriptome assembly, filtering of known genes, and lncRNA prediction. At the end point, users will obtain a set of predicted lncRNAs for downstream use.
著者X L, M N, Z X, TF C, HM L
期刊名稱Methods in Molecular Biology
出版社Humana Press
頁次245 - 255

上次更新時間 2022-14-01 於 23:58