Therapeutic treatment against ocular inflammation by green tea extract and catechins in experimental uveitis
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摘要Introduction and Project Objectives: To determine the treatment effects of green tea extract (GTE) and catechins against ocular inflammation in experimental uveitis models.

Methods.: Endotoxin-induced uveitis {EIU) rat model and experimental autoimmune uveitis (EAU) mouse model. Ocular inflammation in EIU model was assessed by slit lamp photography, whereas that in EAU model was assessed by confocal scanning laser ophthalmoscopy, optical coherence tomography {OCT), fundus fluorescein angiography (FFA) and electroretinography (ERG). Tissue cells and molecules were investigated by confocal microscope, RTPCR, GCMS, and LCMSMS, After EIU induction, 275 and 550 mg/kg GTE were intragastrically fed into the rats 2, 8, 26, 32 hours after LPS injection. For the EAU model, 137.5 and 275 mg/kg GTE or 96.25 and 192.5 mg/kg EGCG catechins were fed once every two days starting from 5 days prior to EAU induction through day 21 post-immunization. The degree of ocular Inflammation, amount of infiltrating cells and ocular histology were evaluated. Expression levels of targeted pro-inflammatory factors were determined in appropriate samples including plasma, humor, ciliary body, and retina tissues. Catechins in tissue cells were quantified for correlations with the molecular effects.
Results: Our results revealed the treatment effects of GTE and catechins on ocular inflammation in two established experimental models and provided new insights into the protective mechanisms of GTE and catechins on uveitis pathogenesis.

Conclusion: Our assessments showed that administration of GTE and catechins alleviated EIU and EAU in morphology and gene expressions, providing evidences for their therapeutic effects in ocular inflammations.
著者Chu WK, Chu KO, Ng TK, Chan SO, Pang CP
會議名稱Health Research Symposium 2019
會議地點Hong Kong

上次更新時間 2020-21-04 於 16:57